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Journal / Bioengineering / Cel-vrije eiwit expressie met behulp van de snel…
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Bioengineering

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JoVE Journal Bioengineering
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Cel-vrije eiwit expressie met behulp van de snel groeiende bacterie Vibrio natriegens

Article DOI: 10.3791/59495-v • 08:52 min • March 14th, 2019
March 14th, 2019 •
1,2, 1, *1, *1,2
1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering
* These authors contributed equally

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Cel-vrije expressiesystemen zijn krachtige en kosten-efficiënte hulpmiddelen voor de synthese van hoog-productie en de screening van belangrijke eiwitten. Hier beschrijven we de voorbereiding van cel-vrije eiwit expressie systeem met behulp van Vibrio natriegens voor de productie van de snelle eiwitten met behulp van de plasmide DNA, lineaire DNA en mRNA sjabloon.

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Bioengineering kwestie 145 Vibrio natriegens ruwe cel extract cel-vrij systeem TX-TL in vitro eiwitsynthese high-throughput scherm
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