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Journal / Bioengineering / Celle-fri Protein udtryk ved hjælp af hurtigt…
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Bioengineering

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JoVE Journal Bioengineering
Celle-fri Protein udtryk ved hjælp af hurtigt voksende bakterie Vibrio natriegens
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Celle-fri Protein udtryk ved hjælp af hurtigt voksende bakterie Vibrio natriegens

Article DOI: 10.3791/59495-v • 08:52 min • March 14th, 2019
March 14th, 2019 •
1,2, 1, *1, *1,2
1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering
* These authors contributed equally

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Celle-fri udtryk systemer er kraftfulde og omkostningseffektive værktøjer til høj overførselshastighed syntese og screening af vigtige proteiner. Her beskriver vi udarbejdelsen af celle-fri protein udtryk system ved hjælp af Vibrio natriegens til hurtig protein produktion ved hjælp af plasmid DNA, lineære DNA og mRNA skabelon.

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Bioteknologi sag 145 Vibrio natriegens rå celle ekstrakt celle-fri system TX-TL in vitro-proteinsyntese høj overførselshastighed skærm
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