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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolatie en kwantitatieve evaluatie van de penseel cellen van muis Tracheas
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Isolatie en kwantitatieve evaluatie van de penseel cellen van muis Tracheas

Article DOI: 10.3791/59496-v • 10:25 min • June 12th, 2019
June 12th, 2019 •
1, 1, 1, 1
1Division of Rheumatology, Allergy and Immunology, Brigham and Women's Hospital, Harvard Medical School

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Penseel cellen zijn zeldzame cholinerge chemosensorische epitheelcellen gevonden in de naïeve muis trachea. Vanwege hun beperkte aantallen is de ex vivo-evaluatie van hun functionele rol in luchtweg-immuniteit en-renovatie een uitdaging. We beschrijven een methode voor isolatie van tracheale penseel cellen door flow cytometrie.

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Immunologie en infectie probleem 148 tracheale borstel cellen cholinerge Epitheelcellen Solitaire chemosensorische cellen bittere smaak sensing cellen bittere smaak receptoren Tuft cellen
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