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Journal / Genetics / CRISPR/Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 核糖核酸蛋白介导的精确基因编辑通过管电穿孔
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Journal (Genetics)
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CRISPR/Cas9 核糖核酸蛋白介导的精确基因编辑通过管电穿孔

Article DOI: 10.3791/59512-v • 08:31 min • June 20th, 2019
June 20th, 2019 •
*1, *1, 2, 1, 1, 1, 1, 1
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Celetrix LLC
* These authors contributed equally

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Click here for the English version.

这里介绍的是使用管电穿孔在哺乳动物细胞中高效CRISPR/Cas9核苷酸介导基因编辑的协议。

Tags

遗传学 问题 148 基因编辑 CRISPR/Cas9 核糖核蛋白 管电穿孔 寡核苷酸 哺乳动物细胞
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