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Biochemistry

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JoVE Journal Biochemistry
Atomabsorptionsspektroskopi til måling af intracellulære zink puljer i pattedyrsceller
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Atomabsorptionsspektroskopi til måling af intracellulære zink puljer i pattedyrsceller

Article DOI: 10.3791/59519-v • 13:04 min • May 16th, 2019
May 16th, 2019 •
*1, *2, 3, 4, 2, 1
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Department of Chemistry, Skidmore College, 3Candiac MR Center, Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
* These authors contributed equally

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Dyrkede primære eller etablerede cellelinjer anvendes almindeligvis til at behandle grundlæggende biologiske og mekanistiske spørgsmål som en indledende tilgang, før der anvendes dyremodeller. Denne protokol beskriver, hvordan man forbereder hele celleekstrakter og subcellulære fraktioner til undersøgelser af zink (Zn) og andre sporstoffer med atomabsorptionsspektroskopi.

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Biokemi zink atomare absorbans spektroskopi proliferation differentiering pattedyr cellekultur subcellulær fraktionering
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