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Immunology and Infection

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JoVE Journal Immunology and Infection
Un modèle de culture de lots in vitro pour estimer les effets des régimes interventionnels sur le microbiote fécal humain
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Un modèle de culture de lots in vitro pour estimer les effets des régimes interventionnels sur le microbiote fécal humain

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Ce protocole décrit un système in vitro de fermentation par lots-culture du microbiote fécal humain, utilisant l'inuline (un prébiotique bien connu et l'un des modulateurs de microbiote les plus largement étudiés) pour démontrer l'utilisation de ce système dans l'estimation des effets de sur la composition du microbiote fécal et les activités métaboliques.

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Immunologie et infection numéro 149 anaérobie culture par lots côlon fermentation in vitro microbiote microflore acides gras à chaîne courte pH
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