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Journal / Immunology and Infection / Un modelo de cultivo por lotes In Vitro para…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Un modelo de cultivo por lotes In Vitro para estimar los efectos de los regímenes intervencionistas en la microbiota fecal humana
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Un modelo de cultivo por lotes In Vitro para estimar los efectos de los regímenes intervencionistas en la microbiota fecal humana

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Este protocolo describe un sistema in vitro de fermentación de cultivo por lotes de microbiota fecal humana, utilizando inulina (un prebiótico bien conocido y uno de los moduladores de microbiota más ampliamente estudiados) para demostrar el uso de este sistema en la estimación de efectos específicos de sobre la composición de la microbiota fecal y las actividades metabólicas.

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Inmunología e infección Número 149 anaeróbico cultivo por lotes colon fermentación in vitro microbiota microflora ácidos grasos de cadena corta pH
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