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Journal / Immunology and Infection / En in vitro-modell för att uppskatta effekterna…
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Immunology and Infection

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JoVE Journal Immunology and Infection
En in vitro-modell för att uppskatta effekterna av interventionella regimer på human fekal Microbiota
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En in vitro-modell för att uppskatta effekterna av interventionella regimer på human fekal Microbiota

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Detta protokoll beskriver ett in vitro-system för jäsning av Human fekal bakterieflora, med hjälp av inulin (en välkänd prebiotika och en av de mest studerade Mikrobiota modulatorer) för att demonstrera användningen av detta system för att uppskatta effekterna av specifika interventioner på fekal bakterieflora sammansättning och metaboliska aktiviteter.

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Immunologi och infektion anaerob sats kultur kolon jäsning in vitro Microbiota mikroflora kortkedjiga fettsyror pH
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