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Journal / Biochemistry / Enkelt trin berigelse af en TAP-Tagged Histon…
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Biochemistry

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JoVE Journal Biochemistry
Enkelt trin berigelse af en TAP-Tagged Histon Deacetylase af Filamentous svamp Aspergillus nidulans for enzymatisk aktivitetsanalyse
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Enkelt trin berigelse af en TAP-Tagged Histon Deacetylase af Filamentous svamp Aspergillus nidulans for enzymatisk aktivitetsanalyse

Article DOI: 10.3791/59527-v • 09:07 min • May 1st, 2019
May 1st, 2019 •
1, 1, 2,3, 1, 1
1Division of Molecular Biology, Biocenter, Medical University of Innsbruck, 2Biology Department, Maynooth University, 3Maynooth University Human Health Research Institute

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Klasse 1 Histon deacetyltransferase (hdacs) som rpda har fået betydning som potentielle mål for behandling af svampeinfektioner. Her præsenterer vi en protokol til den specifikke berigelse af TAP-Tagged RpdA kombineret med en HDAC aktivitet assay, der tillader in vitro effekt testning af Histon histondeacetylase hæmmere.

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Biokemi Histon Deacetylase kromatin enzymaktivitet HDAC-analyse Affinity rensning kromatografi tobak etch virus protease Aspergillus nidulans filamentous svampe enkelt trin protein berigelse multi protein Komplekser
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