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Journal / Biochemistry / 丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定
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Isolation of Single-Fiber from Freeze-Dried Human Skeletal Muscle
High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
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丝状真菌曲霉菌的单步富集蛋白脱乙酰化酶的酶活性测定

Article DOI: 10.3791/59527-v • 09:07 min • May 1st, 2019
May 1st, 2019 •
1, 1, 2,3, 1, 1
1Division of Molecular Biology, Biocenter, Medical University of Innsbruck, 2Biology Department, Maynooth University, 3Maynooth University Human Health Research Institute

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像 RpdA 这样的1级组蛋白去乙酰化酶 (Hdac) 作为治疗真菌感染的潜在靶点变得越来越重要。在这里, 我们提出了一个协议的特普标签 RpdA 结合 HDAC 活性检测, 允许体外疗效测试组蛋白去乙酰化酶抑制剂的方案.

Tags

生物化学 第147期 组蛋白去乙酰化酶、染色质、酶活性、HDAC 测定、亲和纯化、色谱、烟草蚀刻病毒蛋白酶、鼻尿曲霉、丝状真菌、单步浓缩蛋白、多蛋白配合 物
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