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Journal / Biochemistry / Single-Step berikelse av en TAP-Tagged Histone…
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Biochemistry

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JoVE Journal Biochemistry
Single-Step berikelse av en TAP-Tagged Histone deacetylase av trådformede sopp Aspergillus nidulans for enzymatisk aktivitet analysen
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Single-Step berikelse av en TAP-Tagged Histone deacetylase av trådformede sopp Aspergillus nidulans for enzymatisk aktivitet analysen

Article DOI: 10.3791/59527-v • 09:07 min • May 1st, 2019
May 1st, 2019 •
1, 1, 2,3, 1, 1
1Division of Molecular Biology, Biocenter, Medical University of Innsbruck, 2Biology Department, Maynooth University, 3Maynooth University Human Health Research Institute

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Klasse 1 histone deacetylases (Hdac) som RpdA har fått betydning som potensielle mål å behandle fungal infeksjoner. Her presenterer vi en protokoll for den spesifikke berikelse av TAP-Tagged RpdA kombinert med en HDCA aktivitet analysen som gjør at in vitro effekt testing av Histone deacetylase hemmere.

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Biokjemi Histone deacetylase Kromatin enzym aktivitet HDCA analysen affinitet rensing kromatografi tobakk etch virus protease Aspergillus nidulans trådformede sopp Single-Step protein berikelse Multiprotein Komplekser
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