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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid
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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

Article DOI: 10.3791/59532-v • 10:42 min • April 11th, 2019
April 11th, 2019 •
1, 1, 1
1Division of Hematology/Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai

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Summary
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Here, we describe the ex vivo expansion of hematopoietic stem cells from CD34+ cells derived from umbilical cord blood treated with a combination of a cytokine cocktail and VPA. This method leads to a significant degree of ex vivo expansion of primitive HSCs for either clinical or laboratory applications.

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Ex Vivo Expansion Hematopoietic Stem Cells Human Umbilical Cord Blood CD34+ Cells Valproic Acid Treatment Cytokine Cocktail Regenerative Medicine Transplantation Therapy Gene Editing Genetically-modified Cells Cell Separator Device Isolation Of CD-34 Positive Cells Separation Buffer EDTA BSA PBS
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