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Journal / Developmental Biology / Isotrope Licht-Sheet-Mikroskopie und…
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Developmental Biology

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JoVE Journal Developmental Biology
Isotrope Licht-Sheet-Mikroskopie und automatisierte Zelllineage-Analysen zu Katalog-Caenorhabditis elegans Embryogenese mit subzellulärer Auflösung
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Isotrope Licht-Sheet-Mikroskopie und automatisierte Zelllineage-Analysen zu Katalog-Caenorhabditis elegans Embryogenese mit subzellulärer Auflösung

Article DOI: 10.3791/59533-v • 08:16 min • June 6th, 2019
June 6th, 2019 •
1,5, 1,5, 1,5, 1,5, 1,5, 4,5, 4,5, 4,5, 2,5, 2,5, 4,5, 3,5, 1,5,6
1Department of Neuroscience and Department of Cell Biology, Yale University School of Medicine, 2Developmental Biology Program, Sloan Kettering Institute, 3Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling, University of Connecticut Health Center, 4Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 5WormGUIDES.org, 6Instituto de Neurobiología, Recinto de Ciencias Médicas, Universidad de Puerto Rico

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Hier stellen wir einen kombinatorischen Ansatz vor, bei dem hochauflösende Mikroskopie, Rechenwerkzeuge und Einzellen-Beschriftungen in lebenden C. eleganischen Embryonen verwendet werden, um die Einzelzellendynamik während der Neuroentwicklung zu verstehen.

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Entwicklungsbiologie Ausgabe 148 DiSPIM CytoSHOW Dekonvolution SpimFusion StarryNite Visualisierung Linie Neuroentwicklung C.elegans Embryonen
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