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Journal / Biochemistry / Isolering af lipoprotein partikler fra hønseæg…
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Biochemistry

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JoVE Journal Biochemistry
Isolering af lipoprotein partikler fra hønseæg æggeblomme til undersøgelse af bakteriel Patogenchlorid fedtsyre inkorporering i membran Fosfolipids
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Isolering af lipoprotein partikler fra hønseæg æggeblomme til undersøgelse af bakteriel Patogenchlorid fedtsyre inkorporering i membran Fosfolipids

Article DOI: 10.3791/59538-v • 11:59 min • May 15th, 2019
May 15th, 2019 •
1, 2, 1
1Department of Microbiology and Molecular Genetics, Michigan State University, 2Department of Physiology, Michigan State University

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Denne metode giver en ramme for at studere inkorporering af eksogene fedtsyrer fra komplekse vært kilder i bakterielle membraner, især Staphylococcus aureus. For at opnå dette, protokoller for tilsætning af lipoprotein partikler fra hønseæg æggeblomme og efterfølgende fedtsyre profilering af bakterielle fosfolipider udnytter massespektrometri er beskrevet.

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Biokemi Staphylococcus aureus fedtsyre lipoprotein massespektrometri phospholipid æggeblomme lipidomics
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