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JoVE Journal Developmental Biology
Generation af Organoids fra mus ekstrahepatisk galdeveje
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Generation af Organoids fra mus ekstrahepatisk galdeveje

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Denne protokol beskriver produktion af en mus ekstrahepatisk galdegang 3-dimensionel organoid system. Disse biliær organoids kan opretholdes i kultur til at studere cholangiocyte biologi. Biliær organoids express markører for både Stamform og galde celler og er sammensat af polariseret epitelceller.

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Udviklingsmæssige biologi spørgsmålet 146 biliær stamceller cholangiocytes organoids 3-dimensionelle kultur system stof screening cholangiopathies
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