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JoVE Journal Developmental Biology
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Generazione dei Organoids da dotti biliari Extrahepatic Mouse

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Questo protocollo descrive la produzione di un sistema di 3-dimensionale organoid del mouse dei dotti biliari extrahepatic. Questi organoids biliare può essere mantenute in coltura per studiare biologia dei colangiociti. Organoids biliare esprimono marcatori di cellule progenitrici e cellule biliari e sono composte da cellule epiteliali polarizzate.

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Biologia dello sviluppo problema 146 cellule progenitrici biliare colangiociti organoids sistema di coltura 3-dimensionale lo screening di stupefacenti cholangiopathies
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