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Geração de Organoids de vias biliares extra-hepáticas Mouse

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Este protocolo descreve a produção de um sistema de 3-dimensional organoides biliar extra-hepática do mouse. Estes organoids biliares podem ser mantidas em cultura para estudar Biologia cholangiocyte. Organoids biliares expressar marcadores do progenitor e células biliares e são compostos de células epiteliais polarizadas.

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Biologia do desenvolvimento edição 146 células progenitoras biliar cholangiocytes organoids sistema de cultura 3-dimensional triagem de drogas cholangiopathies
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