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JoVE Journal Developmental Biology
Generación de organoides de conductos biliares extrahepáticos del ratón
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Generación de organoides de conductos biliares extrahepáticos del ratón

Article DOI: 10.3791/59544-v • 09:13 min • April 23rd, 2019
April 23rd, 2019 •
1, 1, 1,2, 1,2, 1
1Departments of Internal Medicine, The University of Michigan, 2Molecular & Integrative Physiology, The University of Michigan

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Este protocolo describe la producción de un sistema de 3 dimensiones organoide ratón vía biliar extrahepática. Estos organoides biliares se pueden mantener en cultivo a estudiar biología de cholangiocyte. Organoides biliares expresan marcadores de progenitoras y células biliares y están compuestas de células epiteliales polarizadas.

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Biología del desarrollo número 146 células progenitoras biliar cholangiocytes organoides sistema de 3 dimensiones de la cultura detección de drogas cholangiopathies
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