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Journal / Immunology and Infection / 增殖人类抗原特异性CD4+…
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Immunology and Infection

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JoVE Journal Immunology and Infection
增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Journal (Immunology and Infection)
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Author Spotlight: Detection and Treatment of Helicobacter pylori Infection
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增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量

Article DOI: 10.3791/59545-v • 07:00 min • June 4th, 2019
June 4th, 2019 •
1, 1, 1, 1,2
1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne, St. Vincent's Hospital

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这里介绍的是一个协议,用于测量增殖CD4+ T细胞,以响应抗原蛋白或肽使用染料稀释。这种测定对稀有抗原特异性T细胞特别敏感,可以进行改性,以利于抗原特异性细胞的克隆。

Tags

免疫学和感染,第148期,人类,CD4,增殖,CFSE,敏感,自抗原,克隆,PBMC
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