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Journal / Bioengineering / Utarbeidelse av nøytralt-ladet, pH-responsive…
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Bioengineering

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JoVE Journal Bioengineering
Utarbeidelse av nøytralt-ladet, pH-responsive polymer nanopartikler for cytosolic siRNA levering
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Utarbeidelse av nøytralt-ladet, pH-responsive polymer nanopartikler for cytosolic siRNA levering

Article DOI: 10.3791/59549-v • 09:09 min • May 2nd, 2019
May 2nd, 2019 •
1, 1, 1,2,3
1Department of Chemical Engineering, University of Mississippi, 2Department of BioMolecular Sciences, University of Mississippi, 3Biomedical Engineering Program, University of Mississippi

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Metoder for å forberede og karakterisere fysikalsk egenskaper og bioactivity av nøytralt-ladet, pH-responsive siRNA nanopartikler er presentert. Kriterier for vellykkede siRNA-nanomedisiner som størrelse, morfologi, overflate ladning, siRNA-lasting og gendeaktivering diskuteres.

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Bioteknologi RNA interferens Endosomal Escape Endosomolysis pH-responsive nanoteknologi polymer nanopartikler biomedisinsk teknikk Legemiddellevering kreft
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