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JoVE Journal Bioengineering
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Beredning av Neutrally laddade, pH-lyhörd polymera nanopartiklar för Cytosolic siRNA leverans

Article DOI: 10.3791/59549-v • 09:09 min • May 2nd, 2019
May 2nd, 2019 •
1, 1, 1,2,3
1Department of Chemical Engineering, University of Mississippi, 2Department of BioMolecular Sciences, University of Mississippi, 3Biomedical Engineering Program, University of Mississippi

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Metoder för att bereda och karakterisera de fysikalisk-kemiska egenskaperna och bioaktiviteten hos neutralt laddade, pH-responsiva siRNA-nanopartiklar presenteras. Kriterier för framgångs rika siRNA nanomedicines som storlek, morfologi, ytladdning, siRNA lastning och nedtystning av gener diskuteras.

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Bio teknik RNA-störning Endosomal flykt Endosomolysis pH-responsiv nano teknik polymera nanopartiklar biomedicinsk teknik läkemedels leverans cancer
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