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JoVE Journal Cancer Research
Tröpfchendigital TRAP (ddTRAP)
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Tröpfchendigital TRAP (ddTRAP): Anpassung des Telomere Repeat Amplification Protocol an die Reaktion auf die digitale Polymerase Chain Reaction

Article DOI: 10.3791/59550-v • 06:38 min • May 3rd, 2019
May 3rd, 2019 •
1, 1, 1
1School of Kinesiology, University of Michigan

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Wir haben erfolgreich den Standard-Telomere-Wiederholungsverstärkungsprotokoll (TRAP) umgebaut, der in Tröpfchenspolonen-Kettenreaktionen eingesetzt werden soll. Dieser neue Test, genannt ddTRAP, ist sensibler und quantitativer, was eine bessere Erkennung und statistische Analyse der Telomeraseaktivität in verschiedenen menschlichen Zellen ermöglicht.

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Krebsforschung Ausgabe 147 Telomerase TRAP-Test Tröpfchendigital PCR Krebs enzymatische Aktivitätserkennung Alterung quantitative Biologie
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