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Détection et caractérisation de l'auto-assemblage des protéines in Vivo par la cytométrie des flux
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Détection et caractérisation de l'auto-assemblage des protéines in Vivo par la cytométrie des flux

Article DOI: 10.3791/59577-v • 05:58 min • July 17th, 2019
July 17th, 2019 •
*1, *1, 1, 1, 1,2
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine
* These authors contributed equally

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Cet article décrit un protocole de cytométrie de flux basé sur FRET pour quantifier l'auto-assemblage de protéine dans les deux cellules de S. cerevisiae et de HEK293T.

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Biologie Numéro 149 Cytométrie de flux FRET agrégation de protéines séparation de phase nucléation protéostasie
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