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Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie
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Opsporen en karakteriseren van zelf-assemblage van eiwitten in vivo door flow Cytometrie

Article DOI: 10.3791/59577-v • 05:58 min • July 17th, 2019
July 17th, 2019 •
*1, *1, 1, 1, 1,2
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology, The University of Kansas School of Medicine
* These authors contributed equally

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Dit artikel beschrijft een FRET-based flow cytometrie protocol voor het kwantificeren van eiwit zelf montage in zowel S. cerevisiae en HEK293T cellen.

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Biologie uitgave 149 flow cytometrie FRET eiwit aggregatie fasescheiding nucleatie proteostase
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