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Journal / Genetics / 伦蒂病毒介导基因在低附着板中制备的人类伪性沉默…
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Genetics

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JoVE Journal Genetics
伦蒂病毒介导基因在低附着板中制备的人类伪性沉默
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伦蒂病毒介导基因在低附着板中制备的人类伪性沉默

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

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提出了一种从分散的人类胰岛细胞中产生基因修饰的人类伪胰岛的函数,这种细胞由携带短发核糖核酸(shRNA)的慢病毒转导。该协议利用现成的酶和培养容器,易于执行,并产生适合功能和形态学研究的转基因人类假岛。

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遗传学 问题 147 shRNA 慢病毒 胰岛素分泌 培养 单细胞 重新聚集
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