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Journal / Genetics / Lentiviral vermittelte Gen-Silencing in humanem…
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Genetics

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JoVE Journal Genetics
Lentiviral vermittelte Gen-Silencing in humanem Pseudoislet in niedrigen Befestigungsplatten zubereitet
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Lentiviral vermittelte Gen-Silencing in humanem Pseudoislet in niedrigen Befestigungsplatten zubereitet

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

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Ein Protokoll zur Erstellung genmodifizierter menschlicher Pseudoislets aus dispergierten menschlichen Inselzellen, die durch Lentivirus mit kurzer Haarnadel-RNA (shRNA) transduziert werden, wird vorgestellt. Dieses Protokoll verwendet leicht verfügbare Enzym- und Kulturgefäße, kann leicht durchgeführt werden und produziert genetisch veränderte menschliche Pseudoislets, die für funktionelle und morphologische Studien geeignet sind.

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Genetik Ausgabe 147 shRNA Lentivirus Insulinsekretion Kultur Einzelzelle Reaggregation
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