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Journal / Genetics / Lentiviral mediert gen demping i Human…
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JoVE Journal Genetics
Lentiviral mediert gen demping i Human Pseudoislet forberedt på lav Vedleggs plater
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Lentiviral mediert gen demping i Human Pseudoislet forberedt på lav Vedleggs plater

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

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En protokoll for å skape gen modifiserte menneskelige pseudoislets fra spredte transduced-celler som er blitt presentert av lentivirus som bærer korte hæler RNA (shRNA) presenteres. Denne protokollen utnytter lett tilgjengelig enzym-og kultur fartøy, kan utføres enkelt, og produserer genmodifiserte menneskelige pseudoislets egnet for funksjonelle og morfologiske studier.

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Genetikk shRNA lentivirus insulin sekresjon kultur enkelt celle reaggregasjon
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