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JoVE Journal Genetics
Lentiviral medierad gen ljuddämpning i humant Pseudoholmen beredd i låga fästplattor
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Lentiviral medierad gen ljuddämpning i humant Pseudoholmen beredd i låga fästplattor

Article DOI: 10.3791/59578-v • 11:24 min • May 14th, 2019
May 14th, 2019 •
1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Department of Internal Medicine, Carver College of Medicine, University of Iowa, 2Fraternal Order of Eagles Diabetes Research Center, University of Iowa, 3Roy J. Carver Department of Biomedical Engineering, University of Iowa

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Ett protokoll för att skapa genmodifierade mänskliga pseudoöar från spridda mänskliga öar celler som är sensorik av lentivirus redovisade kort hårnål RNA (shRNA) presenteras. Detta protokoll utnyttjar lättillgängliga enzym-och kultur fartyg, kan utföras enkelt och producerar genetiskt modifierade pseudoöar som lämpar sig för funktionella och morfologiska studier.

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Genetik shRNA lentivirus insulinsekretion kultur enda cell reaggregation
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