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JoVE Journal
Immunology and Infection

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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱
 
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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱

Article DOI: 10.3791/59609-v
July 10th, 2019

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Summary July 10th, 2019

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我们提出了一种协议,用单个T细胞和珠子粒子使原子力显微镜(AFM)的吊带功能化,用于免疫学研究。显示了通过AFM探测单对T细胞树突状细胞结合的过程,并监测宏噬细胞对单个固体颗粒的实时细胞响应,并采用荧光成像。

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