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JoVE Journal
Immunology and Infection

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通过微注射研究3D组织衍生的人体器官培养系统中的隐孢子虫感染
 
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通过微注射研究3D组织衍生的人体器官培养系统中的隐孢子虫感染

Article DOI: 10.3791/59610-v 05:31 min September 14th, 2019
September 14th, 2019

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Summary

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Click here for the English version.

我们描述了用于制备卵母细胞和纯化孢子菌的方案,用于研究隐孢子虫的人类肠道和气道器官的感染。我们演示了将寄生虫微注射到肠道器官流明和有机体免疫染色的程序。最后,我们描述了从器官中分离生成的卵母细胞。

Tags

免疫学和感染,问题151,隐孢子虫,有机物,显微注射,宿主微生物,肠,肺,隐孢子虫病,孢子虫,卵囊虫
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