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Journal / Immunology and Infection / Studere Cryptosporidium infektion i 3D…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Studere Cryptosporidium infektion i 3D vævs-afledte humane Organoid kultur systemer ved mikroinjektion
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Studere Cryptosporidium infektion i 3D vævs-afledte humane Organoid kultur systemer ved mikroinjektion

Article DOI: 10.3791/59610-v • 05:31 min • September 14th, 2019
September 14th, 2019 •
1, 1,3, 2
1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), UMC Utrecht, 2Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, 3Janssen Pharmaceutica

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Vi beskriver protokoller til at forberede oocyster og rense sporozoiter til at studere infektion af humane tarm og luftvejs organoider af Cryptosporidium parvum. Vi demonstrerer procedurerne for mikroinjektion af parasitter i tarm organoid lumen og immun farvning af organoider. Endelig beskriver vi isolationen af genererede oocyster fra organoiderne.

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Immunologi og infektion Cryptosporidium organoider mikroinjektion vært-mikrobe tarm lunge Cryptosporidiose sporozoiter oocyster
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