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Journal / Biochemistry / Karakterisering af proteiner efter…
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Biochemistry

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JoVE Journal Biochemistry
Karakterisering af proteiner efter størrelse-eksklusions kromatografi koblet til multi-vinkel Light spredning (SEC-MALS)
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Karakterisering af proteiner efter størrelse-eksklusions kromatografi koblet til multi-vinkel Light spredning (SEC-MALS)

Article DOI: 10.3791/59615-v • 10:00 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 2
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

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Denne protokol beskriver kombinationen af størrelses udelukkelse kromatografi med multi-vinkel lys spredning (SEC-MALS) for absolut karakterisering af proteiner og komplekser i opløsning. SEC-MALS bestemmer molekylvægten og størrelsen af rene proteiner, indfødte oligomerer, heterokomplekser og modificerede proteiner såsom glycoproteiner.

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Biokemi størrelse-udelukkelse kromatografi multi-vinkel lys spredning (MALS) protein karakterisering molekylvægt kvægserum albumin oligomerer aggregater protein-protein komplekser kvalitetskontrol
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