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Journal / Biochemistry / Karakterisering av proteiner av…
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Biochemistry

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JoVE Journal Biochemistry
Karakterisering av proteiner av storlek-uteslutning kromatografi kopplad till multi-Angle ljus spridning (SEC-MALS)
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Karakterisering av proteiner av storlek-uteslutning kromatografi kopplad till multi-Angle ljus spridning (SEC-MALS)

Article DOI: 10.3791/59615-v • 10:00 min • June 20th, 2019
June 20th, 2019 •
1, 2, 3, 2
1Wyatt Technology Corporation, 2Wolfson Centre for Applied Structural Biology, The Alexander Silberman Institute of Life Science, The Hebrew University of Jerusalem, 3Danyel Biotech Ltd.

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Detta protokoll beskriver kombinationen av storlek uteslutningskromatografi med flera vinklar ljus spridning (SEC-MALS) för absolut karakterisering av proteiner och komplex i lösning. SEC-MALS bestämmer molekyl vikt och storlek på rena proteiner, infödda oligomerer, heterokomplex och modifierade proteiner som glykoproteiner.

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Biokemi storlek-uteslutningskromatografi flervinkelljus spridning (MALS) proteinkarakterisering molekyl vikt bovint serumalbumin oligomerer aggregat protein-proteinkomplex kvalitets kontroll
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