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Journal / Biochemistry / 生体内細胞からのRNA単離に対するリボソーム親和性精製(TRAP)の翻訳…
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Biochemistry
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JoVE Journal Biochemistry
生体内細胞からのRNA単離に対するリボソーム親和性精製(TRAP)の翻訳
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生体内細胞からのRNA単離に対するリボソーム親和性精製(TRAP)の翻訳

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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我々は、RNA精製と組み合わせてリボソーム中の増強された緑色蛍光タンパク質(EGFP)のEC特異的遺伝的タグを介して、マウス脳、肺および心臓組織における血管内皮細胞(EC)からリボソーム結合mRNAを精製するアプローチを提示する。.

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生化学,問題147,血管新生,動脈分化,血管内皮細胞,リボソーム親和性精製の翻訳,RNA抽出,リアルタイムqPCR
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