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Journal / Biochemistry / Endotel hücrelerinden RNA yalıtım için Ribosome…
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Biochemistry
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JoVE Journal Biochemistry
Endotel hücrelerinden RNA yalıtım için Ribosome affinity arıtma (TRAP) çevirisi In vivo
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Endotel hücrelerinden RNA yalıtım için Ribosome affinity arıtma (TRAP) çevirisi In vivo

Article DOI: 10.3791/59624-v • 08:53 min • May 25th, 2019
May 25th, 2019 •
1,2, 3,4, 1,3, 1, 2, 3, 3,4,5
1Blood Research Institute, Blood Center of Wisconsin, 2Department of Medicine, Medical College of Wisconsin, 3Department of Surgery, The University of Alabama at Birmingham, 4Department of Biomedical Engineering, The University of Alabama at Birmingham, 5GBS Program, Graduate School, The University of Alabama at Birmingham

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Eğer RNA arıtma ile birlikte ribozomlarda içinde gelişmiş yeşil floresan proteinin (EGFP) EC özgü genetik etiketi ile doğrudan fare beyin, akciğer ve kalp dokularda vasküler endotel hücrelerden (ECS) ribosome bağlı mRNA arındırmak için bir yaklaşım sunuyoruz .

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Biyokimya sayı 147 anjiyogenez arterioler farklılaşma vasküler endotel hücreler ribozom yakınlık Temizleme tercüme RNA ekstraksiyon gerçek zamanlı qPCR
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