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Journal / Genetics / 用于评估肿瘤细胞中的微RNA水平、功能和相关靶基因的体外协议…
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Genetics

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JoVE Journal Genetics
用于评估肿瘤细胞中的微RNA水平、功能和相关靶基因的体外协议
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用于评估肿瘤细胞中的微RNA水平、功能和相关靶基因的体外协议

Article DOI: 10.3791/59628-v • 09:45 min • May 21st, 2019
May 21st, 2019 •
1, 1, 1, 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

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该协议使用基于探针的实时聚合酶链反应(PCR)、磺胺胺B(SRB)测定、3'未翻译区域(3'UTR)克隆和荧光素酶测定来验证感兴趣的miRNA的目标基因,并了解miRNA的功能。

Tags

遗传学 问题 147 生物 Eukaryota 疾病 肿瘤 化学品和药物 核酸 核苷酸 分析 诊断和治疗技术和设备 治疗 微RNA miRNA-107 实时PCR SRB测定、剂量反应曲线、克隆、荧光素酶测定
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