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JoVE Journal Genetics
En in vitro-protokol til evaluering af MicroRNA-niveauer, funktioner og tilknyttede Målgener i tumor celler
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En in vitro-protokol til evaluering af MicroRNA-niveauer, funktioner og tilknyttede Målgener i tumor celler

Article DOI: 10.3791/59628-v • 09:45 min • May 21st, 2019
May 21st, 2019 •
1, 1, 1, 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

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Denne protokol bruger en Probe-baseret real-time polymerase kædereaktion (PCR), en sulforhodamin B (SRB) assay, 3 ' ikke oversat regioner (3 ' UTR) kloning, og en der analyse for at verificere målgener af en Mirna af interesse og til at forstå funktionerne i Mirnas.

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Genetik organismer Eukaryota sygdomme neoplasmer kemikalier og narkotika nukleinsyrer nukleotider og Nukleosier analytiske diagnostiske og terapeutiske teknikker og udstyr Therapeutics microRNA miRNA-107 real-time PCR SRB analyse dosis-respons kurve kloning luciferaseanalyse
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