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JoVE Journal
Genetics

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Digital PCR-based Competitive Index for High-through Analysis of Fitness in Salmonella Digital PCR-based Competitive Index for High-through Analysis of Fitness in Salmonella Digital PCR-based Competitive Index for High-through analysis of Fitness in Salmonella Digital PCR-based
 
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Digital PCR-based Competitive Index for High-through Analysis of Fitness in Salmonella Digital PCR-based Competitive Index for High-through Analysis of Fitness in Salmonella Digital PCR-based Competitive Index for High-through analysis of Fitness in Salmonella Digital PCR-based

Article DOI: 10.3791/59630-v 07:11 min May 13th, 2019
May 13th, 2019

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Summary

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Click here for the English version.

Cette approche moléculaire pour déterminer la forme physique bactérienne facilite la détection précise et précise des micro-organismes à l'aide de codes-barres d'ADN génomique uniques qui sont quantifiés par l'intermédiaire du PCR numérique. Le protocole décrit le calcul de l'indice concurrentiel des souches de Salmonella; cependant, la technologie est facilement adaptable aux protocoles exigeant la quantification absolue de n'importe quel organisme génétiquement malléable.

Tags

Génétique Numéro 147 Indice concurrentiel facteurs de virulence condition physique quantification bactérienne ddPCR PCR numérique indice concurrentiel annulé Salmonella code à barres d'ADN quantification moléculaire interactions génétiques
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