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JoVE Journal Cancer Research
Vurdering tumor mikromiljø af metastase døråbning-medieret vaskulær permeabilitet forbundet med kræft celle udbredelse ved hjælp af Intravital Imaging og fast vævsanalyse
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Vurdering tumor mikromiljø af metastase døråbning-medieret vaskulær permeabilitet forbundet med kræft celle udbredelse ved hjælp af Intravital Imaging og fast vævsanalyse

Article DOI: 10.3791/59633-v • 09:42 min • June 26th, 2019
June 26th, 2019 •
1,2,3, 1,2,4, 1,2, 1,2, 1,2,4, 1,2,3,4, 1,2,3,5, 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Integrated Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center

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Vi beskriver to metoder til vurdering af forbigående vaskulær permeabilitet forbundet med tumor mikromiljø af metastase (TMEM) døråbning funktion og cancer celle intravasation ved hjælp af intravenøs injektion af høj molekylvægt (155 kDa) dextran i mus. Metoderne omfatter intravital billeddannelse i levende dyr og fast vævsanalyse ved hjælp af immunofluorescens.

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Retraktion intravital billeddannelse immunofluorescens blodkar permeabilitet tumor mikromiljø af metastase (TMEM) høj molekylvægt dextran immunofluorescens metastase
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