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In vivo to-Color 2-Foton Imaging av genetisk-Tagged reporter celler i huden

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Morfologiske endringer skjer i immunrespons Fibroblast celler etter aktivering og fremme endringer i mobilnettet rekruttering. Bruk av 2-Foton Imaging i forbindelse med en genetisk konstruert Fibroblast-spesifikt protein 1 (FSP1)-grobunn; tdTomato floxed-Stop-floxed (TB/TB) muse linje og grønn fluorescensmerkete merket LIPOPOLYSAKKARID-FITC, kan vi illustrere svært spesifikke opptak av lipopolysakkarid i dermal fibroblaster og morfologiske endringer i vivo.

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Bioteknologi multi-Foton Imaging toll-lignende reseptor 4 genetisk reporter lipopolysakkarid in vivo
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