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In Vivo 2-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Article DOI: 10.3791/59647-v • 05:45 min • July 11th, 2019
July 11th, 2019 •
1, 1, 1, 1
1School of Brain and Behavioral Science, Center for Advanced Pain Studies, University of Texas at Dallas

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Los cambios morfológicos ocurren en las células de fibroblastos inmune sensibles después de la activación y promueven alteraciones en el reclutamiento celular. Utilización de imágenes de 2 fotones junto con una proteína específica de fibroblastos de ingeniería genética 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) línea de ratón y verde fluorescently etiquetado lipopolysaccharide-FITC, podemos ilustrar la absorción altamente específica de lipopolisacárido en fibroblastos dérmicos y cambios morfológicos in vivo.

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Bioingeniería Número 149 Imágenes multifotona receptor similar al peaje 4 genético reportero lipopolisacárido in vivo
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