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Journal / Genetics / Designing automatisert, høy gjennomstrømming,…
JoVE Journal
Genetics

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JoVE Journal Genetics
Designing automatisert, høy gjennomstrømming, kontinuerlig cellevekst eksperimenter bruke eVOLVER
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Designing automatisert, høy gjennomstrømming, kontinuerlig cellevekst eksperimenter bruke eVOLVER

Article DOI: 10.3791/59652-v • 07:26 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,3, 1,2, 4, 1,2,5
1Biological Design Center, Boston University, 2Department of Biomedical Engineering, Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, 4Department of Bioengineering, Rice University, 5Wyss Institute for Biologically Inspired Engineering, Harvard University

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Den eVOLVER rammeverket muliggjør høy gjennomstrømming kontinuerlig mikrobiell kultur med høy oppløsning og dynamisk kontroll over eksperimentelle parametere. Denne protokollen demonstrerer hvordan du bruker systemet til å gjennomføre en kompleks fitness eksperiment, guiding brukere på programmering automatisert kontroll over mange individuelle kulturer, måling, innsamling, og samspill med eksperimentelle data i sanntid.

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Genetikk eVOLVER kontinuerlig kultur mikrobielle populasjoner Lab automatisering vekst utvalg eksperimentell evolusjon fitness landskap systemer biologi
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