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Journal / Developmental Biology / MRNA Electroporation kullanarak Avian embriyoları …
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Developmental Biology

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JoVE Journal Developmental Biology
MRNA Electroporation kullanarak Avian embriyoları çoklu proteinlerin hızlı ve verimli Ifade
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MRNA Electroporation kullanarak Avian embriyoları çoklu proteinlerin hızlı ve verimli Ifade

Article DOI: 10.3791/59664-v • 09:40 min • June 7th, 2019
June 7th, 2019 •
1, 2,3, 1,2,3
1Department of Biological Sciences, University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute, Children's Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology, University of Southern California, Los Angeles

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Biz bıldırcın embriyo modeli sistemde çoklu proteinlerin hızlı ve verimli ifade izin veren bir yöntem olarak haberci RNA (mRNA) elektroporasyonu rapor. Bu yöntem floresan hücreler için kullanılabilir ve Elektroporasyon kısa bir süre sonra zaman atlamalı mikroskopinin in vivo hareketlerini kaydetmek.

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Gelişimsel biyoloji Sayı 148 mRNA Electroporation zaman-lapse mikroskobu bıldırcın kuş embryo FRAP
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