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Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
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Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019
August 14th, 2019 •
1,2,3, 4, 5, 2, 6, 3, 3, 3, 1, 2, 1,2,3
1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6People's Hospital of Longhua

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Summary
Automatic Translation

An effective enzymatic method for isolation of primary porcine aortic endothelial cells (pAECs) from miniature pigs is described. The isolated primary pAECs can be used to investigate the immune and coagulation response in xenotransplantation.

Tags

Isolation Culture Primary Aortic Endothelial Cells Miniature Pigs Xenotransplantation Technique Purified Endothelium Cells Porcine Abdominal Wall Medical Alcohol Iodine Heparin Sodium Catheter Diaphragm Left Ventricle Ribs Heart And Lungs Washing Buffer
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