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Journal / Cancer Research / Micromanipulatie van circulerende tumor cellen…
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Cancer Research

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JoVE Journal Cancer Research
Micromanipulatie van circulerende tumor cellen voor downstream moleculaire analyse en gemetastaseerde potentiële beoordeling
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Micromanipulatie van circulerende tumor cellen voor downstream moleculaire analyse en gemetastaseerde potentiële beoordeling

Article DOI: 10.3791/59677-v • 05:17 min • May 14th, 2019
May 14th, 2019 •
*1, *1, *1, 1,2, 1
1Cancer Metastasis Laboratory, Department of Biomedicine, University of Basel and University Hospital Basel, 2SIB Swiss Institute of Bioinformatics
* These authors contributed equally

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Hier presenteren we een geïntegreerde workflow om fenotypische en moleculaire kenmerken te identificeren die circulerende tumorcellen (CTCs) karakteriseren. Wij combineren levende immunokleuring en robotachtige micromanipulatie van enige en geclusterde CTCs met enige cel-gebaseerde technieken voor stroomafwaartse analyse en beoordeling van metastasen-zaait capaciteit.

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Kankeronderzoek circulerende tumorcellen circulerende tumor cel clusters micromanipulatie circulerende tumor cel cultuur enkele cel sequencing metastase borstkanker
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