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Journal / Bioengineering / Standardisierter und skalierbarer Assay zur…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Standardisierter und skalierbarer Assay zur Untersuchung perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
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Standardisierter und skalierbarer Assay zur Untersuchung perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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Diese Methode beschreibt die Kultur von iPSC-abgeleiteten Endothelzellen als 40 durchfundierte 3D-Mikrogefäße in einer standardisierten mikrofluidischen Plattform. Diese Plattform ermöglicht die Untersuchung von gradientengetriebenen angiogenen Keimen in 3D, einschließlich Anastomose und Stabilisierung der angiogenen Sprossen in einer skalierbaren und hochdurchsatzigen Weise.

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Bioengineering Ausgabe 153 3D-Zellkultur Mikrofluidik in vitro humaninduzierte pluripotente Stammzellen Endothelzellen Angiogenese Gradienten Hochdurchsatz-Screening Assay-Entwicklung
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