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Journal / Bioengineering / Ensayo estandarizado y escalable para estudiar la …
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Bioengineering

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JoVE Journal Bioengineering
Ensayo estandarizado y escalable para estudiar la brotación angiogénica 3D perfundida de células endoteliales derivadas de iPSC in Vitro
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Ensayo estandarizado y escalable para estudiar la brotación angiogénica 3D perfundida de células endoteliales derivadas de iPSC in Vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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Este método describe el cultivo de células endoteliales derivadas de iPSC como 40 microvas 3D perfundidas en una plataforma microfluídica estandarizada. Esta plataforma permite el estudio de la brotación angiogénica impulsada por gradientes en 3D, incluyendo anastomosis y estabilización de los brotes angiogénicos de una manera escalable y de alto rendimiento.

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Bioingeniería Número 153 cultivo de células 3D microfluidos in vitro células madre pluripotentes inducidas por humanos células endoteliales angiogénesis gradientes cribado de alto rendimiento desarrollo de ensayos
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