Chapters
Summary
This method describes the culture of iPSC-derived endothelial cells as 40 perfused 3D microvessels in a standardized microfluidic platform. This platform enables the study of gradient-driven angiogenic sprouting in 3D, including anastomosis and stabilization of the angiogenic sprouts in a scalable and high-throughput manner.
Tags
Standardized Scalable Assay Study Perfused 3D Angiogenic Sprouting IPSC-derived Endothelial Cells In Vitro New Targets Angiogenesis Assay Cellular Micro Environments Gradients Perfusion High Throughput Screening Health Disease Cancers Tissues Repair Cardiovascular Diseases Tissue Regeneration Microfluidic Platform Law Of Volumes Pipette Microscope Plate Upside Down Microfluidic Channels Hold The Plate Wendy Stam Technician Transfer Microfluidic 384-well Plate Sterile Laminar Flow Hood
PLAYLIST
Behavior
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Research • BehaviorNovel Object Recognition Test for the Investigation of Learning and Memory in Mice -
Research • BehaviorNovel Object Recognition and Object Location Behavioral Testing in Mice on a Budget -
Research • BehaviorAssessment and Evaluation of the High Risk Neonate: The NICU Network Neurobehavioral Scale
Biochemistry
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Research • BiochemistryA Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation -
Research • BiochemistryPurification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion -
Research • BiochemistryCharacterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Bioengineering
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Research • BioengineeringOperation of a Benchtop Bioreactor -
Research • BioengineeringStandardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro -
Research • BioengineeringElectric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Biology
Cancer Research
Chemistry
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Research • ChemistryConducting Miller-Urey Experiments -
Research • ChemistryUtilization of Stop-flow Micro-tubing Reactors for the Development of Organic Transformations -
Research • ChemistryMatrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Developmental Biology
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Research • Developmental BiologyThawing, Culturing, and Cryopreserving Drosophila Cell Lines -
Research • Developmental BiologyIsolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta -
Research • Developmental BiologyDifferentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Engineering
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Research • EngineeringA Polymer-based Piezoelectric Vibration Energy Harvester with a 3D Meshed-Core Structure -
Research • EngineeringThree-electrode Coin Cell Preparation and Electrodeposition Analytics for Lithium-ion Batteries -
Research • EngineeringCarrier Lifetime Measurements in Semiconductors through the Microwave Photoconductivity Decay Method
Environment
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Research • EnvironmentProtocol for Microplastics Sampling on the Sea Surface and Sample Analysis -
Research • EnvironmentIsolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments -
Research • EnvironmentSampling Soils in a Heterogeneous Research Plot
Genetics
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Research • GeneticsCRISPR Guide RNA Cloning for Mammalian Systems -
Research • GeneticsPromoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions -
Research • GeneticsTargeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Immunology and Infection
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Research • Immunology and InfectionProduction of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting -
Research • Immunology and InfectionA Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells -
Research • Immunology and InfectionViral Concentration Determination Through Plaque Assays: Using Traditional and Novel Overlay Systems
Medicine
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Research • MedicineA Case Series of Successful Abdominal Closure Utilizing a Novel Technique Combining a Mechanical Closure System with a Biologic Xenograft that Accelerates Wound Healing -
Research • MedicineRemote Laboratory Management: Respiratory Virus Diagnostics -
Research • MedicineSingle-stage Dynamic Reanimation of the Smile in Irreversible Facial Paralysis by Free Functional Muscle Transfer
Neuroscience
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Research • NeuroscienceWhole Animal Perfusion Fixation for Rodents -
Research • NeuroscienceCompartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips -
Research • NeuroscienceUse of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
