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Journal / Bioengineering / 标准化和可扩展的测定，以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
标准化和可扩展的测定，以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽
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标准化和可扩展的测定，以研究iPSC衍生内皮细胞在Vitro的渗透3D血管生成发芽

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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该方法将iPSC衍生内皮细胞的培养描述为标准化微流体平台中的40个注入3D微容器。该平台支持在3D中研究梯度驱动的血管生成，包括以可扩展的高通量方式血管生成芽的血管生成和稳定。

Tags

生物工程，第153期，3D细胞培养，微流体，体外，人类诱导多能干细胞，内皮细胞，血管生成，梯度，高通量筛选，测定发展
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