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Preparação de Cryo-seções retinal do rato para immunohistochemistry

Article DOI: 10.3791/59683-v • 05:25 min • July 1st, 2019
July 1st, 2019 •
1, 2, 2, 1
1Department of Biomedical Sciences, University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine, University of Pennsylvania School of Veterinary Medicine

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Este relatório descreve métodos detalhados para preparar seções congeladas do retina do rato para immunohistochemistry (IHC). Os métodos descritos incluem dissecção do copo posterior ocular, fixação paraformaldeído, incorporação em meios de temperatura de corte ideal (OCT) e orientação tecidual, corte e imunocoloração.

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Neurociência retina Cryo-seção ótima temperatura de corte de mídia OCT imuno-histoquímica mouse Eye Enucleação
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